Composite

Part:BBa_K146001:Design

Designed by: Dave Westenberg   Group: iGEM08_Missouri_Miners   (2008-10-29)


AOX1 ethanol sensitive promoter cloned into RFP vector BBa_J61002


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3802
    Illegal XbaI site found at 3817
    Illegal SpeI site found at 877
    Illegal PstI site found at 1760
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3802
    Illegal SpeI site found at 877
    Illegal PstI site found at 1760
    Illegal NotI site found at 1753
    Illegal NotI site found at 3808
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3802
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3802
    Illegal XbaI site found at 3817
    Illegal SpeI site found at 877
    Illegal PstI site found at 1760
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3802
    Illegal XbaI site found at 3817
    Illegal SpeI site found at 877
    Illegal PstI site found at 1760
    Illegal AgeI site found at 1456
    Illegal AgeI site found at 1568
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2841


Design Notes

No special considerations are required.


Source

The AOX1 promoter was PCR amplified from pPCK3.

References